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worthington 胸腺组织分离指南

发布者:艾美捷科技    发布时间:2022-06-10     
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原代细胞分离中,针对胸腺涉及到树突状,胸腺基质细胞、脂肪干细胞、基质细胞,上皮,胸腺等等的解离通常会使用酶,如何选择合适的酶来完成对应胸腺的解离,以及原代细胞采集的常规操作, 艾美捷针对胸腺相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:胸腺组织分离指南.png

 

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

 

胸腺
种属种属详情细胞名称酶组合推荐,含推荐浓度培养基参考文献
Human树突状 / DentriticCollagenase Type 2: 0.1%;
Deoxyribonuclease I: 0.002%
RPMI 1640Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations, Blood 97, 1733-41, 2001
小鼠Mouse胸腺基质细胞 / Thymic stromalCollagenase Type 4: 0.02%;
Neutral Protease: 0.02%;
Deoxyribonuclease I: 0.0025%
RPMI-1640Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells., Proc Natl Acad Sci U S A 109, 3463-8, 2012
小鼠Mouse基质细胞 / StromalCollagenase Type 3: 0.2%;
Hyaluronidase: 0.1%
RPMI 1640Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution, J Immunol , 4869, 2004
小鼠Mouse树突状 / DentriticCollagenase Type 3: 0.1%;
Deoxyribonuclease I: 325 u/ml
RPMI 1640Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
小鼠Mouse, 3-6 week胸腺 / ThymicCollagenase Type 3: 100-400 u/mlHBSSSmith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance, Int Immunol 9, 1355-65, 1997
小鼠Mouse (BALB/c, C3H, C57BL/6), 1-28 day上皮,胸腺 / Epithelial, thymusNeutral Protease: 1.5 痢/mlDMEMRopke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium, In Vitro Cell Dev Biol 26, 671, 1990
小鼠Mouse, C3H, 16 wk old, female上皮 / EpithelialCollagenase Type 3: 0.1%DMEMEhmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture, In Vitro Cell Dev Biol 22 (12), 738, 1986
小鼠Mouse, Swiss, 6 wk胸腺 / ThymusCollagenase Type 3: 150 u/mlDMEMJones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures, In Vitro 17, 431, 1981
大鼠Rat, postnatal胸腺 / ThymicTrypsin: 0.05%HBSSBonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells., Nature 466, 978, 2010
大鼠Rat胸腺上皮 / Thymic epithelialCollagenase Type 3: 0.1%DMEMMasuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 21C:4.1, 1995
大鼠Rat (ACI/NMs X BUF/Mna) F1, male, 28 months ;Rat, (ACI/MNs) male, 8 weeks (also bovine, adult)上皮 / EpthelialCollagenase Type 3: 0.1%Eagle's MEM Serum-freeMasuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990


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