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worthington 干细胞组织分离指南

发布者:艾美捷科技    发布时间:2022-06-09     

原代细胞分离中,针对干细胞涉及到基质细胞血管,羊膜间充质干细胞、脂肪干细胞、产后牙周韧带,滑膜间充质干细胞等等的解离通常会使用酶,如何选择合适的酶来完成对应干细胞的解离,以及原代细胞采集的常规操作, 艾美捷针对干细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:干细胞组织分离指南.png


本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南


犬/狗23-33 kg基质细胞血管 / Stromal vascularCollagenase: see referenceMedia 199EWilliams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013
犬/狗Canine羊膜间充质干细胞 / Amniotic membrane MSCTrypsin: 0.25%;
Collagenase Type 1: 0.2%
LG-DMEMPark, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012
犬/狗Canine, 20-25 kg脂肪干细胞 / Adipose stem cellsCollagenase: see referenceMedia-199Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
Equine脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%PBSVidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008
Equine, 1-5 year脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%PBSVidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007
Human脂肪来源的干细胞 / Adipose derived stemCollagenase Type 4: 150-300 u/mlRPMI-1640Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019
Human产后牙周韧带 / Postnatal periodontal ligamentCollagenase Type 1: 0.01%DMEM/F12Yam, G., Teo, E., Setiawan, M., Lovatt, M., Yusoff, N., Fuest, M., Goh, B. and Mehta, J.: Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy., J Cell Mol Med , , 2018
Human沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromalCollagenase Type 2: 270 u/mlDMEM/F12Oppliger, B., Joerger-Messerli, M., Simillion, C., Mueller, M., Surbek, D. and Schoeberlein, A.: Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact., Cytotherapy 19, 829-838, 2017
Human脐带间充质基质细胞 / Umbilical cord mesenchymal stromalCollagenase Type 1: 0.2%;
Hyaluronidase: 0.1%;
Deoxyribonuclease I: 0.01%
MEMde Witte, S., Lambert, E., Merino, A., Strini, T., Douben, H., O'Flynn, L., Elliman, S., de Klein, A., Newsome, P., Baan, C. and Hoogduijn, M.: Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion., Cytotherapy 19, 798-807, 2017
Human滑膜间充质干细胞 / Synovial mesenchymal stemCollagenase: 0.3%MEMMatsumura, E., Tsuji, K., Komori, K., Koga, H., Sekiya, I., Muneta, T.: Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells., Cytotherapy 19, 181-193, 2017
Human脂肪来源的间充质干细胞 / Adipose derived mesenchymal stemCollagenase animal free: 0.1%PBSClark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017
Human运动神经元 / Motor neuronsPapain: 20 u/mlEBSSRigamonti, A., Repetti, G., Sun, C., Price, F., Reny, D., Rapino, F., Weisinger, K., Benkler, C., Peterson, Q., Davidow, L., Hansson, E. and Rubin, L.: Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System., Stem Cell Reports 6, 993-1008, 2016
Human沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromalCollagenase Type 2: 0.6%DMEMLim, J., Razi, Z., Law, J., Nawi, A., Idrus, R. and Ng, M.: MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord., Cytotherapy 18, 1493-1502, 2016
Human脂肪干细胞 / Adipose stemCollagenase Type 1: 0.1%DMEM/F12Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016
Human腱干细胞 / Tendon stemCollagenase Type 1: 0.3%;
Neutral Protease: 0.4%
MEMRandelli, P., Menon, A., Ragone, V., Creo P., Alfieri, M., Perucca, O., Banfi, G., Cabitza, P., Tettamanti, G. and Anastasia, L.: Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study., BMC Complement Altern Med 16, 293, 2016
Human间充质基质细胞 / Mesenchymal stromalCollagenase Type 4: 0.2%PBSChoudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015
Human脂肪干细胞 / Adipose derived stem cellsCollagenase Type 2: 0.1%HBSSSatish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015
Human牙间充质干细胞 / Dental mesenchymal stemCollagenase Type 1: 0.3%;
Neutral Protease: 0.4%
DMEMWu, Z., Wang, J., Dong, R., Wang, L., Fan, Z., Liu, D. and Wang, S.: Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells., Int J Clin Exp Med 8, 7220-30, 2015
Human蜕膜间充质干细胞 / Decidua mesenchymal stemTrypsin: 0.25%;
Collagenase Type 1: 1.0%;
Deoxyribonuclease I: 0.005%
PBSKusuma, G., Menicanin, D., Gronthos, S., Manuelpillai, U., Abumaree, M., Pertile, M., Brennecke, S. and Kalionis, B.: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua., PLoS ONE 10, e0141246, 2015
Human骨骼肌干细胞 / Skeletal muscle stemCollagenase Type 2: 750 u/ml;
Neutral Protease: 2 u/ml
Ham's F10Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells., Stem Cell Reports 5, 621, 2015
Human脊髓神经祖细胞 / Spinal cord neural progenitorPDS kit: with modificationsEBSSMothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
Human华顿果冻间充质干细胞 / Wharton jelly mesenchymal stemCollagenase Type 2: 0.1%;
Trypsin NF 1:250: 0.25%
PBSLee, S., Park, B., Kim, J., Jekarl, D., Choi, H., Lee, S., Kim, M., Kim, Y. and Park, M.: The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells., Cytotherapy 17, 1723-31, 2015
Human, adult脂肪来源的间充质干细胞 / Adipose derived mesenchymal stemCollagenase Type 2: 0.1%see referenceAl-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014
Human, adult脂肪来源的干细胞 / Adipose derived stemCollagenase Type 1: 0.15%DMEMKoellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014
Human脐间充质干细胞 / Umbilical mesenchymal stemCollagenase Type 2: 0.1%;
Trypsin: 0.25%
DMEMHang, H., Yu, Y., Wu, N., Huang, Q., Xia, Q. and Bian, J.: Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction., PLoS ONE 9, e104133, 2014

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