Unicode编码转换 - 站长工具

欢迎您访问武汉艾美捷科技有限公司官方网站!
站点地图 服务热线:400-6800-868
  • 产品
  • 文章
AGENT BRAND 品牌专题

Cell Biolabss:氧自由基抗氧化能力(ORAC)活性检测试剂盒

发布者:艾美捷科技    发布时间:2021-10-28     
分享到:

自在基与多种不同我们皮肤疾病的發生发展壮大都具有干系密切的干系。在去除自在基的流程中,抗老化反应性物剂激发着更核心的功用。之所以,熟知 抗老化反应性物剂的功用及技能就让人觉得至关更核心。

 在测评抗空气腐蚀物专业专业功能的很多技术中,抗空气腐蚀专业专业功能ORAC(俗称,氧什么是自由基溶解专业专业功能,Oxygen Radical Absorbance Capacity)讲解法,被介定为在线检测抗空气腐蚀物抗空气腐蚀专业专业功能的的标准规定技术,是现阶段常见被展开的有一个的标准规定测评有效途径。根据该法更具相当人体顺利要求、实操简简单单、认为度和灵活度高、为准性和显现性佳、mtk量、不要受人为原则原则干涉等益处,被诸多用在畜牧业,美食,营养品品,医疗药品及医学界区域。 

自由基.jpg

 

那么如何分析样本中氧自由基抗氧化能力/氧自由基吸收能力(ORAC)?作为专业的生命科学医药原料供应商,艾美捷科技为您推荐:众多文章引用的,氧自由基抗氧化能力(ORAC)活性检测试剂盒

产品氧自由基抗氧化能力(ORAC)检测试剂盒
OxiSelect ORAC Activity Assay Kit
货号CBL-STA-345
检测方法荧光法
适用样本类型血清/血浆,尿液,细胞裂解液/提取物,组织裂解物,营养样品(食品,植物等等)
说明书下载 (请于实验前,详细阅读说明书
试剂盒原理ORAC分析法中的自由基主要来源于偶氮化合物2,2'-偶氮-双-(2-眯基丙烷)氯化二氢(2,2'-azobis(2-amidinopropane)dihydrochloride,AAPH)热分解产生的过氧化氢自由基,也可以是芬顿(Fenton)反应过程中产生的羟自由基,以荧光素钠(Sodium Flourescein,FL)为荧光探针,观察自由基与荧光探针作用后,探针荧光强度的衰退过程,以水溶性维生素E类似物(Trolox)作为抗氧化标准物质,检测体系中各种抗氧化剂延缓探针荧光强度衰退的能力,以此评价抗氧化剂的抗氧化能力。
实验优势1 简单快捷:不到2小时完成实验;
2 样本适用:多样性
3 提供标准品;
试剂盒组分1. 96-well Microtiter Plate
2. Fluorescein Probe (100X)
3. Free Radical Initiator
4. Antioxidant Standard (Trolox)
5. Assay Diluent (4X)
保存条件收到后,Fluorescent Probe和Antioxidant Standard (Trolox) 保存于-20℃;其它所有试剂组分保存在4℃。

* 本产品仅适用于科研用途.

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒,原理与标曲展示:

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图

 

FAQ:

 1.能否能否测试测试冷藏留存的样品英文?RE:常见符合于在 -80℃ 下保持两根月元样本,但让我们意见和建议尽或许测试仪鲜新样本以收获较好报告 2.ORAC、HORAC和TAC检侧内的差别,怎么样去 采用?RE:每个三种类型测量方法都测量方法抗硫化业务业务技能,但略微与众不同。ORAC检验工具分折氧人身政治权利自在基的抗硫化业务业务技能;HORAC检验工具分折羟基人身政治权利自在基的抗硫化业务业务技能;TAC立于单电子无线转到(SET)体系和铜亚铁离子,检验工具总体设计布局抗硫化业务业务技能。总体设计布局一般说来,研究方案工作员更风险偏好选用ORAC分折而就不是HORAC,毕竟过氧人身政治权利自在基或许比羟基人身政治权利自在基更有关于。 

3.更多常见问题,请点击:cellbiolabs.com/faq/oxidative-stress-faq/orac-assay

 

近期发表文章(仅摘取部分展示):

Rolnik, A. et al. (2021). Antioxidant and hemostatic properties of preparations from Asteraceae family and their chemical composition - Comparative studies. Biomed Pharmacother. 142:111982. doi: 10.1016/j.biopha.2021.111982.Xiao, L. et al. (2021). Hydrogen-Generating Silica Material Prevents UVA-ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents. Antioxidants (Basel). 10(1):E76. doi: 10.3390/antiox10010076.Yuasa, M. et al. (2020). Antioxidant Activities and Taste Qualities of Fresh Onions Produced in Minamishimabara City, Nagasaki, Japan. J Food Sci Technol. doi: 10.3136/fstr.26.167.Gómez-Zorita, S. et al. (2020). Comparative Effects of Pterostilbene and Its Parent Compound Resveratrol on Oxidative Stress and Inflammation in Steatohepatitis Induced by High-Fat High-Fructose Feeding. Antioxidants (Basel). 9(11):E1042. doi: 10.3390/antiox9111042.Yuasa, M. et al. (2020). Antioxidant and taste properties of fresh onion (Allium cepa L.) leaves. J. Food Meas. Charact. doi: 10.1007/s11694-020-00704-w.Wake, H. et al. (2020). Histidine-rich glycoprotein possesses anti-oxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction. Free Radic Res. doi: 10.1080/10715762.2020.1825703.Suruga, K. et al. (2020). Soybean fermentation with basidiomycetes (medicinal mushroom mycelia). Chem. Biol. Technol. Agric. 7:23. doi: 10.1186/s40538-020-00189-1.Cheng, H.S. et al. (2020). Pleiotrosepic ameliorative effects of ellagitannin geraniin against metabolic syndrome induced by high-fat diet in rats. Nutrition. doi: 10.1016/j.nut.2020.110973.Jeon, S.Y. et al. (2020). Combined Extract of Vitis vinifera L. and Centella asiatica Synergistically Attenuates Oxidative Damage Induced by Hydrogen Peroxide in Human Umbilical Vein Endothelial Cells. Prev Nutr Food Sci. 25(2):173-183. doi: 10.3746/pnf.2020.25.2.173.Xiao, L. et al. (2020). Enzyme-digested Colla Corii Asini (E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells. Neural Regen Res. 15:2270-2. doi: 10.4103/1673-5374.285000.Yang, Y. et al. (2020). Chemical and cytological evaluation of honeybee pollen antioxidant ability. J Food Sci. doi: 10.1111/1750-3841.15047.Hu, R. et al. (2020). Antioxidant Performances of Corn Gluten Meal and DDGS Protein Hydrolysates in Food, Pet Food, and Feed Systems. Journal of Agriculture and Food Research. doi: 10.1016/j.jafr.2020.100030.Liu, Y.H. et al. (2020).  Acetylcholinesterase inhibitory activity and neuroprotection in vitro, molecular docking, and improved learning and memory functions of demethylcurcumin in scopolamine-induced amnesia ICR mice. Food Funct. doi: 10.1039/C9FO02339A.Kim, D. et al. (2020). Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice. Food Sci Anim Resour. doi: 10.5851/kosfa.2020.e3.Mishima, E. et al. (2019). Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. pii: ASN.2019060570. doi: 10.1681/ASN.2019060570.Riquelme, S. et al. (2019). Bench-scale extraction of stilbenoids and other phenolics from stored grape canes (Vitis Vinifera):  Optimization process, chemical characterization, and potential protection against oxidative damage.  Journal of the Chilean Chemical Society. 64(2).Liu, Y.H. et al. (2019). Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in D-galactose-induced BALB/c mice in vivo. Bot Stud. 60(1):9. doi: 10.1186/s40529-019-0258-x.Engstr m- st, J. et al. (2019). Eco-physiological responses of copepods and pteropods to ocean warming and acidification. Sci Rep. 9(1):4748. doi: 10.1038/s41598-019-41213-1.Johnson, M. et al. (2019). Influence of Green Leafy Vegetables in Diets with an Elevated ω-6:ω-3 Fatty Acid Ratio on Rat Blood Pressure, Plasma Lipids, Antioxidant Status and Markers of Inflammation. Nutrients. 11(2). pii: E301. doi: 10.3390/nu11020301.Statland, J.M. et al. (2019). Rasagiline for amyotrophic lateral sclerosis: A randomized, controlled trial. Muscle Nerve. 59(2):201-207. doi: 10.1002/mus.26335.kerstrm, B. et al. (2019).  rA1M-035, a Physicochemically Improved Human Recombinant α1-Microglobulin, Has Therapeutic Effects in Rhabdomyolysis-Induced Acute Kidney Injury. Antioxid Redox Signal. 30(4):489-504. doi: 10.1089/ars.2017.7181.Bednarek, N. et al. (2018). El Nio-Related Thermal Stress Coupled With Upwelling-Related Ocean Acidification Negatively Impacts Cellular to Population-Level Responses in Pteropods Along the California Current System With Implications for Increased Bioenergetic Costs. Frontiers in Marine Science. 5:1-17. doi:10.3389/fmars.2018.00486.Watanabe, S. et al. (2018). Detection of betacyanin in red-tube spinach (Spinacia oleracea) and its biofortification by strategic hydroponics. PLoS One. 13(9):e0203656. doi: 10.1371/journal.pone.0203656.Hinman, A. et al. (2018). Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase. PLoS One. 13(8):e0201369. doi: 10.1371/journal.pone.0201369.Gregory Rivera, M. et al. (2018). Peptide Inhibitor of Complement C1 (PIC1) demonstrates antioxidant activity via single electron transport (SET) and hydrogen atom transfer (HAT). PLoS One. 13(3):e0193931. doi: 10.1371/journal.pone.0193931.Ferrare, K. et al. (2018). Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory ( Cichorium intybus L.) for an antidiabetic effect. Journal of Ethnopharmacology. 215:241–248. doi:10.1016/j.jep.2017.12.035.Lee, J.K. et al. (2018). Alleviation of ascorbic acid-induced gastric high acidity by calcium ascorbate in vitro and in vivo. Korean J Physiol Pharmacol. 22(1):35-42. doi: 10.4196/kjpp.2018.22.1.35.Sheng Cheng, H. (2017). The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome. Nutrients. 9(9): 984. doi: 10.3390/nu9090984.Alvarenga, I.C. et al. (2017). 239 Effect of diets containing sorghum fractions on antioxidant capacity of dogs and phenolic acids of both food and plasma measured by high performance liquid chromatography (HPLC). J. Anim. Sci. 95(Suppl4):117-1.Smith, S.C. (2017). 237 Development of a preference ranking test with dogs. J. Anim. Sci. 95(Suppl4):116-117. doi: 10.2527/asasann.2017.237.................................................................................................看过那里,您心跳哪天?以后保持联系小艾吧!
  • 高品质保障 最新的生育科研系统
  • 高性价比 价宜优质,性价变高
  • 高效省心 从购置到适用,靠得住无优
  • 安全运输 建全的自我保护具体措施健康装卸搬运

联系方式微信扫一扫一直在线客服咨询中心

微信营销网络咨询
全国各地兔费枝术扶持 400-6800-868
百度在线电话客服