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Cell Biolabs:氧自由基抗氧化能力(ORAC)检测试剂盒

发布者:艾美捷科技    发布时间:2021-10-27     
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自由基与多种人类疾病的发生发展都有着密切的关系。在清除自由基的过程中,抗氧化剂发挥着重要的作用。因此,了解抗氧化剂的作用及能力就变得至关重要。

 在考核抗硫化剂技能的这些的办法中,抗硫化技能ORAC(又名,氧人身自由基吸引技能,Oxygen Radical Absorbance Capacity)分析一下法,被鉴定为论文检测抗硫化剂抗硫化技能的规定的办法,是当今普通被展开的个规定考核的方法。因此该法存在将近有机体生活必要条件、使用非常简单、承认度和精确度度高、准确度性和逆转性佳、高通骁龙量、更易受人工影响因素干拢等特点,被比较广泛广泛用于农林,食物,保健养生品,制剂及医学检验方向。 

自由基.jpg

 

那么如何分析样本中氧自由基抗氧化能力/氧自由基吸收能力(ORAC)?作为专业的生命科学医药原料供应商,艾美捷科技为您推荐:众多文章引用的,氧自由基抗氧化能力(ORAC)活性检测试剂盒

产品氧自由基抗氧化能力(ORAC)检测试剂盒
OxiSelect ORAC Activity Assay Kit
货号CBL-STA-345
检测方法荧光法
适用样本类型血清/血浆,尿液,细胞裂解液/提取物,组织裂解物,营养样品(食品,植物等等)
说明书下载 (请于实验前,详细阅读说明书
试剂盒原理ORAC分析法中的自由基主要来源于偶氮化合物2,2'-偶氮-双-(2-眯基丙烷)氯化二氢(2,2'-azobis(2-amidinopropane)dihydrochloride,AAPH)热分解产生的过氧化氢自由基,也可以是芬顿(Fenton)反应过程中产生的羟自由基,以荧光素钠(Sodium Flourescein,FL)为荧光探针,观察自由基与荧光探针作用后,探针荧光强度的衰退过程,以水溶性维生素E类似物(Trolox)作为抗氧化标准物质,检测体系中各种抗氧化剂延缓探针荧光强度衰退的能力,以此评价抗氧化剂的抗氧化能力。
实验优势1 简单快捷:不到2小时完成实验;
2 样本适用:多样性
3 提供标准品;
试剂盒组分1. 96-well Microtiter Plate
2. Fluorescein Probe (100X)
3. Free Radical Initiator
4. Antioxidant Standard (Trolox)
5. Assay Diluent (4X)
保存条件收到后,Fluorescent Probe和Antioxidant Standard (Trolox) 保存于-20℃;其它所有试剂组分保存在4℃。

* 本产品仅适用于科研用途.

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒,原理与标曲展示:

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-原理示意图

 

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图.jpg

氧自由基抗氧化能力(ORAC)活性检测试剂盒-标曲示意图

 

FAQ:

 1.需不需要可能检测速冻同步保存的打样定制?RE:一般来说适于于在 -80℃ 下保持三十一个月之内样机,但咱们建立尽将会测试软件新鲜的样机以提升更优报告单 2.ORAC、HORAC和TAC检则相互的有别,怎么样才能进行?RE:各种三种类型估测都估测清除优质基物性能素质,但偶有多种。ORAC探测具体解析氧优质基的清除优质基物性能素质;HORAC探测具体解析羟基优质基的清除优质基物性能素质;TAC鉴于单電子更改(SET)机能和铜亚铁离子,探测整体清除优质基物性能素质。整体来讲,探索职工更个性化取舍ORAC具体解析而并非是HORAC,而是过氧优质基仍然比羟基优质基更相应的。 

3.更多常见问题,请点击:cellbiolabs.com/faq/oxidative-stress-faq/orac-assay

 

近期发表文章(仅摘取部分展示):

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