Unicode编码转换 - 站长工具

欢迎您访问武汉艾美捷科技有限公司官方网站!
站点地图 服务热线:400-6800-868
  • 产品
  • 文章
AGENT BRAND 品牌专题

Cell Biolabs:His标签蛋白ELSIA检测试剂盒

发布者:艾美捷科技    发布时间:2021-10-28     
分享到:
His标鉴,也被视为多组氨酸标鉴(polyhistidine),His6标鉴或hexa组氨酸标鉴,这就是本身在转染的神经细胞中指标蛋清的C互联网或N端上由也至少要6个组氨酸残基外链上所构成的氨基字段。 

His标签蛋白定量.jpg

 His-Tag是迄今为止宽泛应用于纯化的凝固标签贴中宽泛应用最宽泛的特征:a:元素较短可至于血清的N端一些pc端b:对蛋白酶的效果及上中游软件后果小c:免疫系统原性低,可以和多性子并用d:纯化形式特别单纯便利许多魔抗使His商品标价签变成 资产重组蛋清酶制作和分离出来的时兴商品标价签。虽然,相对于未纯化的印刷品,His商品标价签蛋清酶的更准一定量印刷品还是很麻烦。 

作为专业的生命科学医药原料供应商,Cell Biolabs中国区金牌代理,艾美捷科技为您推荐:His-Tag 蛋白ELISA 试剂盒,这是一种基于竞争性ELISA测定法,专为快速检测和定量His标签蛋白样品(C 端或 N 端)而开发。

货号AKR-130
名称His-Tag Protein ELISA Kit
His标签蛋白ELSIA检测试剂盒
目的His标签蛋白进行定量检测
原理将含有His标签的样品或含有His标签的重组蛋白标准品添加到已经包被好多组氨酸的微孔板上。短暂孵育后,加入抗 6xHis单克隆抗体,然后加入HRP偶联的二抗。未知样品中的His标签蛋白含量是通过与由预先确定的His标签蛋白标准品制备的标准曲线进行比较来确定的。
结果His标签蛋白标准曲线:

His标签蛋白定量.jpg


N端His标签蛋白定量检测结果.png

说明书
 调用论文资料:1.Helalat, S.H. et al. (2020). Investigating the efficacy of UVSE protein at repairing CPD and 6–4 pp DNA damages in human cells. J Photochem Photobiol B. doi: 10.1016/j.jphotobiol.2020.111843.2.Huang, Y. et al. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nanosapper potentiates immunotherapy against immune-excluded tumors. Nat Commun. 11(1):622. doi: 10.1038/s41467-020-14425-7.3.Karamitros, C.S. et al. (2020). Bacterial expression systems for enzymatic activity in droplet-based microfluidics. Anal Chem. doi: 10.1021/acs.analchem.9b04969.4.Arnett, C. et al. (2019). Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Appl Microbiol Biotechnol. doi: 10.1007/s00253-019-10141-5.5.Liu, Q. et al. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma. ACS Nano. 12(2):1250-1261. doi: 10.1021/acsnano.7b07384.6.Ibarra-Sánchez, L.A. et al. (2018). Antimicrobial behavior of phage endolysin PlyP100 and its synergy with nisin to control Listeria monocytogenes in Queso Fresco. Food Microbiol. 72:128- 134. doi: 10.1016/j.fm.2017.11.013.7.Reyes-Corona, D. et al. (2017). Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion. PLoS One. 12(11):e0188239. doi: 10.1371/journal.pone.0188239.8.Warnock, N.D. et al. (2017). Nematode neuropeptides as transgenic nematicides. PLoS Pathog. 13(2):e1006237. doi: 10.1371/journal.ppat.1006237.9.Rinaldo, A. R. et al. (2015). A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins. Plant Physiol. 169:1897-916.10.Akiyama, Y. et al. (2014). The identification of affinity peptide ligands specific to the variable region of human antibodies. Biomed Res. 35:105-116.11.Dong, Y. et al. (2013). HMGB1 protein does not mediate the inflammatory response in spontaneous spinal cord regeneration: a hint for CNS regeneration. J. Biol. Chem. 288:18204- 182128. 找到这块,您一见钟情什么时间?立即搞好关系小艾吧!
Unicode编码转换 - 站长工具相关的文章
  • 高品质保障 为先进的制作研发团队枝术
  • 高性价比 价宜优质,而且性价比低
  • 高效省心 从购卖到动用,信任有保障
  • 安全运输 成熟的保护预防措施预防措施安会运输业

微信营销扫码支付再线电话客服

腾讯微信网络咨询
山东省兔费系统能够 400-6800-868
网络qq客服